6OG8

Crystal structure of Green Fluorescent Protein (GFP); S65T, H148D with globally incorporated 3-F1Y; circular permutant (50-51)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.127
Spacegroup name	P 1 21 1
Unit cell lengths	50.073, 70.072, 61.072
Unit cell angles	90.00, 95.93, 90.00

Refinement procedure

Resolution	27.283 - 1.599
R-factor	0.1604
Rwork	0.159
R-free	0.19040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zf3
RMSD bond length	0.008
RMSD bond angle	1.046
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.283	1.656
High resolution limit [Å]	1.599	1.599
Rmerge	0.068	0.603
Rmeas	0.081	0.793
Rpim	0.043	0.506
Number of reflections	54415	4373
<I/σ(I)>	14.17	1.37
Completeness [%]	97.2	78.6
Redundancy	3.5	2.3
CC(1/2)	0.998	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	50 mM sodium acetate, pH 5.0, 100 mM NaCl, 25% PEG 3350