6OG4

plasminogen binding group A streptococcal M protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	173
Detector technology	PIXEL
Collection date	2018-02-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	52.599, 52.599, 198.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.460 - 1.700
R-factor	0.2038
Rwork	0.203
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dur
RMSD bond length	0.006
RMSD bond angle	0.859
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.460	1.760
High resolution limit [Å]	1.699	1.699
Rmerge	0.026	0.236
Rmeas	0.036	0.334
Number of reflections	31726	3048
<I/σ(I)>	15
Completeness [%]	99.7
Redundancy	2
CC(1/2)	0.998	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M HEPES, pH 7.0, 2 M ammonium sulfate