6OFU
X-ray crystal structure of the YdjI aldolase from Escherichia coli K12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-16 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.763, 85.663, 84.268 |
| Unit cell angles | 90.00, 109.88, 90.00 |
Refinement procedure
| Resolution | 34.740 - 1.750 |
| R-factor | 0.14342 |
| Rwork | 0.141 |
| R-free | 0.18156 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gvf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.443 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.071 | 0.213 |
| Number of reflections | 104686 | 7205 |
| <I/σ(I)> | 33.2 | 4.4 |
| Completeness [%] | 96.5 | 90.6 |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 10-12% PEG5000 100 mM MES 5 mM DHAP |
