6OFR
The crystal structure of the outer membrane transporter YddB from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.987 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 76.520, 76.520, 412.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.920 - 2.400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zgv |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.920 | 2.480 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.219 | 1.960 |
Rpim | 0.072 | 0.622 |
Number of reflections | 49176 | 4454 |
<I/σ(I)> | 6 | 1.5 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 10.4 | 10.7 |
CC(1/2) | 0.991 | 0.722 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 293 | 0.1 M Na cacodylate, 0.15 M Ca acetate, 15 % PEG 8000 and 20 % glycerol |