6OFN
Crystal structure of green fluorescent protein (GFP); S65T, T203(3-OMeY); ih circular permutant (50-51)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.190, 67.763, 58.849 |
| Unit cell angles | 90.00, 101.51, 90.00 |
Refinement procedure
| Resolution | 38.742 - 1.649 |
| R-factor | 0.1637 |
| Rwork | 0.162 |
| R-free | 0.19090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zf3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.781 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13rc2_2986: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.742 | 1.710 |
| High resolution limit [Å] | 1.649 | 1.650 |
| Rmeas | 0.075 | 1.480 |
| Number of reflections | 43982 | 4295 |
| <I/σ(I)> | 21.3 | 1.5 |
| Completeness [%] | 98.1 | 96.3 |
| Redundancy | 13.6 | 12.9 |
| CC(1/2) | 1.000 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.15 M ammonium acetate, 34% PEG 3350 |
