6OFM

Crystal structure of green fluorescent protein (GFP); S65T, Y66(3-CH3Y); ih circular permutant (50-51)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	50.977, 68.810, 60.608
Unit cell angles	90.00, 101.58, 90.00

Refinement procedure

Resolution	36.264 - 1.480
R-factor	0.1605
Rwork	0.160
R-free	0.17790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zf3
RMSD bond length	0.006
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.260	1.530
High resolution limit [Å]	1.480	1.480
Rmeas	0.074	1.440
Number of reflections	66961	6627
<I/σ(I)>	19.7	1.5
Completeness [%]	97.9	97.2
Redundancy	13.8	13.6
CC(1/2)	1.000	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.15 M ammonium acetate, 28% PEG 3350