6OFK
Crystal structure of green fluorescent protein (GFP); S65T; ih circular permutant (50-51)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.8856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.937, 68.887, 60.948 |
Unit cell angles | 90.00, 100.71, 90.00 |
Refinement procedure
Resolution | 36.490 - 1.150 |
R-factor | 0.15108 |
Rwork | 0.150 |
R-free | 0.17760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zf3 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.672 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0241) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.490 | 1.190 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmeas | 0.035 | 1.760 |
Number of reflections | 146731 | 14174 |
<I/σ(I)> | 28.4 | 1.4 |
Completeness [%] | 98.0 | 95.3 |
Redundancy | 13.5 | 12.2 |
CC(1/2) | 1.000 | 0.781 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.15 M ammonium acetate, 34% PEG 3350 |