6OFK
Crystal structure of green fluorescent protein (GFP); S65T; ih circular permutant (50-51)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.937, 68.887, 60.948 |
| Unit cell angles | 90.00, 100.71, 90.00 |
Refinement procedure
| Resolution | 36.490 - 1.150 |
| R-factor | 0.15108 |
| Rwork | 0.150 |
| R-free | 0.17760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zf3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0241) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.490 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmeas | 0.035 | 1.760 |
| Number of reflections | 146731 | 14174 |
| <I/σ(I)> | 28.4 | 1.4 |
| Completeness [%] | 98.0 | 95.3 |
| Redundancy | 13.5 | 12.2 |
| CC(1/2) | 1.000 | 0.781 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.15 M ammonium acetate, 34% PEG 3350 |
