6OFJ
Cryo-EM structure of the native rhodopsin dimer from rod photoreceptor cells
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Phase Plate | Volta phase plate |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.5 |
Software | RELION,SerialEM |
Symmetry | C2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |