6OF6

Crystal structure of tRNA^ Ala(GGC) bound to cognate 70S A-site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	211.260, 452.330, 626.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.534 - 3.200
R-factor	0.197
Rwork	0.195
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.534	44.534	3.130
High resolution limit [Å]	3.050	13.650	3.050
Rmerge	0.193	0.057	0.940
Rmeas	0.209	0.062	1.190
Total number of observations	6185978
Number of reflections	998253	11003	46971
<I/σ(I)>	7.48	22.27	0.9
Completeness [%]	88.9	82.3	56.9
Redundancy	6.197	6.333	2.08
CC(1/2)	0.992	0.993	0.290

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP