6OEI

Yeast Spc42 N-terminal coiled-coil fused to PDB: 3K2N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97929
Spacegroup name	C 2 2 21
Unit cell lengths	49.664, 193.108, 95.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.378 - 2.580
R-factor	0.2101
Rwork	0.208
R-free	0.24380
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CRANK2
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.570
High resolution limit [Å]	2.550	8.720	2.550
Rmerge	0.074	0.045	0.537
Rmeas	0.077	0.047	0.562
Rpim	0.021	0.014	0.162
Number of reflections	14732	419	353
<I/σ(I)>	10.7
Completeness [%]	99.9	96.3	99.4
Redundancy	13.8	11.6	11.3
CC(1/2)		0.999	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	Crystals were grown from a 1:1 mixture of 16 mg/ml protein solution containing 20 mM HEPES pH7.6, 100 mM NaCl and well solution containing 100 mM MOPS pH 7.0, 5% (w/v) PEG4K, 5%(w/v) MPD, and 150 mM NaCitrate.