6OEC
Yeast Spc42 Trimeric Coiled-Coil Amino Acids 181-211 fused to PDB: 3H5I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-13 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97962 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 133.197, 169.723, 122.028 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.142 - 2.514 |
R-factor | 0.1976 |
Rwork | 0.195 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h5i |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.094 | 0.063 | 0.261 |
Rmeas | 0.101 | 0.067 | 0.307 |
Rpim | 0.034 | 0.021 | 0.156 |
Total number of observations | 527628 | ||
Number of reflections | 88519 | 9641 | 8157 |
<I/σ(I)> | 5.3 | ||
Completeness [%] | 94.3 | 98.1 | 88.1 |
Redundancy | 6 | 9.1 | 2.9 |
CC(1/2) | 0.998 | 0.636 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | Crystals grown from 1:1 mixture of 12 mg/ml protein stored in 20 mM HEPES pH 7.6, 100 mM NaCl and a well solution consiting of 100 mM PIPES pH 6.0, 10% (w/v) MEPEG 5K, 700 mM tetramethylamine chloride |