6OEC

Yeast Spc42 Trimeric Coiled-Coil Amino Acids 181-211 fused to PDB: 3H5I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-13
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97962
Spacegroup name	P 21 21 2
Unit cell lengths	133.197, 169.723, 122.028
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.142 - 2.514
R-factor	0.1976
Rwork	0.195
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h5i
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.094	0.063	0.261
Rmeas	0.101	0.067	0.307
Rpim	0.034	0.021	0.156
Total number of observations	527628
Number of reflections	88519	9641	8157
<I/σ(I)>	5.3
Completeness [%]	94.3	98.1	88.1
Redundancy	6	9.1	2.9
CC(1/2)		0.998	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	Crystals grown from 1:1 mixture of 12 mg/ml protein stored in 20 mM HEPES pH 7.6, 100 mM NaCl and a well solution consiting of 100 mM PIPES pH 6.0, 10% (w/v) MEPEG 5K, 700 mM tetramethylamine chloride