6ODG

SVQIVY, Crystal Structure of a tau protein fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	93
Detector technology	PIXEL
Collection date	2017-09-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	4.710, 37.500, 21.110
Unit cell angles	90.00, 92.95, 90.00

Refinement procedure

Resolution	18.750 - 1.000
R-factor	0.2469
Rwork	0.245
R-free	0.26630
Structure solution method	AB INITIO PHASING
RMSD bond length	0.015
RMSD bond angle	1.337
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.750	18.750	1.030
High resolution limit [Å]	1.000	4.470	1.000
Rmerge	0.319	0.223	0.754
Rmeas	0.337	0.232	0.831
Total number of observations	26400
Number of reflections	3392	27	186
<I/σ(I)>	3.57	8.18	1.12
Completeness [%]	85.2	65.9	64.8
Redundancy	7.783	7.926	5.011
CC(1/2)	0.985	0.989	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		291	3.3 mg/mL SVQIVY, 0.667 M DL-malic acid, pH 7.0, 8% w/v PEG3350