6OD2

Yeast Spc42 C-terminal Antiparallel Coiled-Coil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-08
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97915
Spacegroup name	P 65 2 2
Unit cell lengths	36.499, 36.499, 206.004
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.609 - 2.435
R-factor	0.2174
Rwork	0.215
R-free	0.26410
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CRANK2
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.120	0.079	0.494
Rmeas	0.124	0.082	0.521
Rpim	0.028	0.019	0.156
Number of reflections	3577	245	150
<I/σ(I)>	7.1
Completeness [%]	99.4	97.2	97.4
Redundancy	18.7	17.2	8.7
CC(1/2)		0.998	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	Crystals were grown from a 1:1 mixture of 12 mg/ml protein stored in 20 mM HEPES pH 7.6, 100 mM NaCl, 0.5 mM TCEP and a well solution containing 80 mM MES pH 6.0, 60% (w/v) MPD, 200 mM NaCl.