6OCR
Crystal structure of human KCTD16 T1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.139, 68.646, 131.679 |
| Unit cell angles | 102.11, 97.98, 89.88 |
Refinement procedure
| Resolution | 41.970 - 2.280 |
| R-factor | 0.2546 |
| Rwork | 0.254 |
| R-free | 0.26980 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 220.000 | 220.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 4.850 | 2.250 |
| Rmerge | 0.041 | 0.023 | 0.617 |
| Total number of observations | 152071 | ||
| Number of reflections | 78702 | 8348 | 3417 |
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 92.0 | 98 | 40 |
| Redundancy | 1.9 | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 293.15 | 20% ethanol, 0.1 M Tris, pH 7.0 |
