6OCP

Crystal structure of a human GABAB receptor peptide bound to KCTD16 T1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	56.792, 69.105, 137.889
Unit cell angles	102.19, 94.83, 91.15

Refinement procedure

Resolution	67.110 - 2.350
R-factor	0.2225
Rwork	0.222
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ocr
RMSD bond length	0.009
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	Aimless (0.2.17)
Phasing software	MOLREP
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.110	2.710
High resolution limit [Å]	2.350	2.350
Rmerge	0.049	0.296
Rmeas	0.070	0.418
Rpim	0.049	0.296
Total number of observations	149442
Number of reflections	77129	26932
<I/σ(I)>	7.4
Completeness [%]	90.7	90.3
Redundancy	1.9	1.9
CC(1/2)	0.991	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293.15	2% PEG 6000 2% glycerol 8% PEG 400 0.1 M sodium cacodylate trihydrate, pH 5.6