6OCG

Crystal structure of VASH1-SVBP complex bound with EpoY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	70.671, 126.454, 46.082
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.121 - 1.833
R-factor	0.1792
Rwork	0.177
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ocf
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.830	4.970	1.830
Rmerge	0.056	0.031	3.541
Rmeas	0.058	0.032	3.681
Rpim	0.016	0.009	0.994
Number of reflections	37002	2039	1811
<I/σ(I)>	50.2
Completeness [%]	99.6	99.5	99.8
Redundancy	13.1	12.2	13.3
CC(1/2)		0.998	0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	5% (v/v) TacsimateTM, 0.1 M HEPES, pH 7.0, and 10% (w/v) polyethylene glycol monomethyl ether 5,000