6OCF
The crystal structure of VASH1-SVBP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 100.558, 100.558, 206.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.214 - 2.102 |
R-factor | 0.1972 |
Rwork | 0.196 |
R-free | 0.22200 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
Rmerge | 0.088 | 0.041 | 2.568 |
Rmeas | 0.090 | 0.042 | 2.625 |
Rpim | 0.018 | 0.009 | 0.528 |
Number of reflections | 48368 | 1721 | 1476 |
<I/σ(I)> | 42.8 | ||
Completeness [%] | 99.6 | 99.7 | 97 |
Redundancy | 24.5 | 24.4 | 20.4 |
CC(1/2) | 0.999 | 0.547 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 0.1 M sodium citrate tribasic dihydrate, pH 5.0, and 18% (w/v) PEG20000 |