6OCE
Structure of the rice hyperosmolality-gated ion channel OSCA1.2
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.2 |
| Energy Filter | GIF Bioquantum |
| Phase Plate | VOLTA PHASE PLATE |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.9 |
| Number of Particle | 169655 |
| Software | RELION,cryoSPARC,Leginon,PHENIX |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
