6OC8

Crystal structure of a VHH against the capsid protein from BLV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	C 2 2 21
Unit cell lengths	120.560, 120.720, 69.941
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.360 - 2.109
R-factor	0.2107
Rwork	0.205
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xt1
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.360	60.360	2.220
High resolution limit [Å]	2.110	6.670	2.110
Rmerge	0.138	0.056	0.666
Rmeas	0.153	0.063	0.740
Rpim	0.065	0.027	0.315
Total number of observations	152335	4853	22554
Number of reflections	29590	1019	4280
<I/σ(I)>	8.7	20.2	2.3
Completeness [%]	99.3	98.4	99.9
Redundancy	5.1	4.8	5.3
CC(1/2)	0.995	0.997	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	2.00 M ammonium sulfate, 7% isopropanol, 15% pentaerythritol ethoxylate