6OC7
HMP42 Fab in complex with Protein G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 91.132, 54.388, 103.081 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 48.103 - 1.296 |
| R-factor | 0.1686 |
| Rwork | 0.168 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fqq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.067 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.103 | 1.370 |
| High resolution limit [Å] | 1.296 | 1.296 |
| Number of reflections | 120493 | 16114 |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 95.9 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 17% PEG 4000, 15% glycerol, 8.5% 2-propanol and 85 mM HEPES pH 7.5 |
