6OC3

Crystal structure of FluA-20 Fab in complex with the head domain of H1 (A/Solomon Islands/3/2006)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	78
Detector technology	PIXEL
Collection date	2016-10-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03315
Spacegroup name	P 21 21 21
Unit cell lengths	100.468, 109.775, 146.394
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.168 - 2.850
R-factor	0.2375
Rwork	0.237
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4YJZ and 6OBZ
RMSD bond length	0.011
RMSD bond angle	1.601
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rpim	0.050	0.310
Number of reflections	36821	1436
<I/σ(I)>	20.6	2
Completeness [%]	95.7	74.6
Redundancy	6.8	5.2
CC(1/2)	1.000	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.12	0.1 M phosphate-citrate, pH 4.2 40%(v/v) PEG300