6OBT
Structural insights into dehydratase substrate selection for the borrelidin and fluvirucin polyketide synthases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2017-05-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.210, 63.350, 40.670 |
| Unit cell angles | 90.00, 100.44, 90.00 |
Refinement procedure
| Resolution | 30.890 - 1.800 |
| Rwork | 0.203 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ln9 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15rc3_3435) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.900 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 24737 | |
| Completeness [%] | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 0.20 M MgCl2, 0.10 M Tris pH 8.5, and 29% (w/v) PEG 4000 |
