6OBL

JAK2 JH2 in complex with JAK168

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97910
Spacegroup name	P 1 21 1
Unit cell lengths	43.605, 57.782, 61.009
Unit cell angles	90.00, 110.71, 90.00

Refinement procedure

Resolution	57.067 - 2.061
R-factor	0.1996
Rwork	0.198
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5usz
RMSD bond length	0.006
RMSD bond angle	0.808
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	2.190
High resolution limit [Å]	2.060	2.060
Rmeas	0.118
Number of reflections	17135	2489
<I/σ(I)>	9.86
Completeness [%]	96.9
Redundancy	5
CC(1/2)	0.996	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1M TRIS, PH 8.0 0.2M SODIUM ACETATE, 0.001 M TCEP, 12-24% PEG 4,000