6OBI
Remarkable rigidity of the single alpha-helical domain of myosin-VI revealed by NMR spectroscopy
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | 900-MHz Bruker Avance-II | 900 | |
| 2 | Bruker | 800-MHz Bruker | 800 | |
| 3 | Bruker | 600-MHz Bruker | 600 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 4D 1H-15N-15N-1H HMQC-NOESY-TROSY-HSQC |
| 10 | 1 | 2 | Heteronuclear TROSY-based relaxation |
| 11 | 5 | 1 | ARTSY |
| 2 | 1 | 1 | TROSY-HSQC |
| 3 | 1 | 1 | 3D TROSY-HNCACB |
| 4 | 1 | 2 | ARTSY-J |
| 5 | 2 | 2 | WEX-III TROSY |
| 6 | 3 | 1 | ARTSY |
| 7 | 3 | 1 | E.COSY-HSQC-TROSY |
| 8 | 4 | 3 | ARTSY |
| 9 | 1 | 2 | Heteronuclear TROSY-based relaxation |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.3 | 1 | 293 |
| 2 | 7.8 | 1 | 293 |
| 3 | 6.3 | 1 | 293 |
| 4 | 6.3 | 1 | 293 |
| 5 | 6.3 | 1 | 293 |
Conformers
| Conformers Calculated Total Number | 500 |
| Conformers Submitted Total Number | 10 |
