6OAW

Crystal structure of a CRISPR Cas-related protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97868
Spacegroup name	P 1 21 1
Unit cell lengths	76.768, 95.294, 77.337
Unit cell angles	90.00, 112.76, 90.00

Refinement procedure

Resolution	39.500 - 2.200
R-factor	0.2133
Rwork	0.211
R-free	0.24670
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.576
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.650	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.077	1.371
Rmeas	0.083	1.487
Rpim	0.032	0.569
Number of reflections	59861	4627
<I/σ(I)>	16
Completeness [%]	99.2	98.7
Redundancy	6.7	6.6
CC(1/2)	0.999	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M ammonium acetate, 0.015 M magnesium acetate, 0.05 M sodium cacodylate, 10% v/v 2-propanol