6OAO
Structure of DBP in complex with human neutralizing antibody 092096
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2017-06-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 130.658, 89.563, 193.705 |
| Unit cell angles | 90.00, 108.05, 90.00 |
Refinement procedure
| Resolution | 19.971 - 3.497 |
| R-factor | 0.2396 |
| Rwork | 0.237 |
| R-free | 0.27890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nuv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.777 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.971 | 3.700 |
| High resolution limit [Å] | 3.497 | 3.497 |
| Rmerge | 0.132 | 0.630 |
| Number of reflections | 53871 | 11515 |
| <I/σ(I)> | 4.91 | |
| Completeness [%] | 92.6 | |
| Redundancy | 1.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 290 | 12% w/v PEG 6,000, 0.1 M MES, pH 6.0 |
