6OAM

Crystal Structure of ChlaDUB2 DUB domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	193
Detector technology	PIXEL
Collection date	2016-12-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 43
Unit cell lengths	108.692, 108.692, 62.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.609 - 2.503
R-factor	0.2843
Rwork	0.283
R-free	0.30570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mrn 1ubq
RMSD bond length	0.020
RMSD bond angle	2.442
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.610	2.610
High resolution limit [Å]	2.500	2.500
Rmerge	0.074
Rpim	0.031
Number of reflections	25243	3083
<I/σ(I)>	22.22
Completeness [%]	99.8
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M HEPES sodium pH 7.5, 0.8 M sodium phosphate monobasic monohydrate, 0.8 M sodium phosphate monobasic, 0.1M Cesium chloride