6OAJ

HUaE34K 19bp SYM DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.115820
Spacegroup name	P 21 21 21
Unit cell lengths	64.602, 86.353, 91.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.492 - 4.092
R-factor	0.3138
Rwork	0.312
R-free	0.32870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yex
RMSD bond length	0.010
RMSD bond angle	1.275
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.492	4.238
High resolution limit [Å]	4.092	4.092
Rmerge	0.119	1.942
Rmeas	0.132	2.129
Rpim	0.055
Number of reflections	4331	406
<I/σ(I)>	7.28	1.01
Completeness [%]	99.3	98.54
Redundancy	6.2	6
CC(1/2)	0.996	0.479

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1M Na-malonate, pH 5.0, 12% PEG 3350