6OAE
Crystal Structure of Human Mucin-like Protocadherin EC1-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 50.427, 78.379, 121.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.610 - 1.900 |
| R-factor | 0.19887 |
| Rwork | 0.197 |
| R-free | 0.23414 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uz8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.531 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.074 | 0.263 |
| Number of reflections | 19398 | 943 |
| <I/σ(I)> | 46.14 | 14.5 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 24.1 | 21.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 277 | 0.1M Sodium Acetate pH 4.4, 0.7M Calcium Chloride |
