6OAA
Cdc48-Npl4 complex processing poly-ubiquitinated substrate in the presence of ADP-BeFx, state 1
EM specimen
Vitrification | Yes |
Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
Microscope | FEI TALOS ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.1 |
Software | cryoSPARC,SerialEM |
CTF correction | PHASE FLIPPING ONLY |