6OA8
Superfolder Green Fluorescent Protein with 4-cyano-L-phenylalanine at the chromophore (position 66)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 55.266, 55.266, 166.483 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.450 - 1.370 |
R-factor | 0.1627 |
Rwork | 0.161 |
R-free | 0.18850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.130 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 165.760 | 1.390 |
High resolution limit [Å] | 1.370 | 1.370 |
Number of reflections | 55127 | 2740 |
<I/σ(I)> | 20.7 | 1.2 |
Completeness [%] | 99.6 | 96.1 |
Redundancy | 13.4 | 7.6 |
CC(1/2) | 0.999 | 0.393 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.1 M Tris-HCl, 2.0 M ammonium sulfate, pH 8.5 |