6O9H
Mouse ECD with Fab1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-06-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.078, 48.974, 150.908 |
| Unit cell angles | 90.00, 101.08, 90.00 |
Refinement procedure
| Resolution | 19.934 - 2.100 |
| R-factor | 0.2054 |
| Rwork | 0.203 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.633 |
| Data reduction software | XDS |
| Data scaling software | SCALA (0.3.6) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.530 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.079 | 0.556 |
| Rmeas | 0.093 | 0.653 |
| Rpim | 0.048 | 0.340 |
| Total number of observations | 226792 | |
| Number of reflections | 61740 | 4399 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 98.7 | 95.4 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.997 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% PEG10000, 0.1 M sodium citrate, pH 5.0 and 15% isopropanol |
