6O8G

Crystal structure of UvrB bound to fully duplex DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979200
Spacegroup name	P 21 21 2
Unit cell lengths	170.685, 201.181, 62.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.240 - 2.640
R-factor	0.2228
Rwork	0.220
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.780
High resolution limit [Å]	2.630	2.630
Number of reflections	64310	9258
<I/σ(I)>	18.9
Completeness [%]	99.8
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, pH 7.5, 0.02 M magnesium chloride, 20-22% poly(acryl acid sodium salt) 5100