6O8A

Thaumatin native-SAD structure determined at 5 keV from microcrystals

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	2.479
Spacegroup name	P 41 21 2
Unit cell lengths	57.698, 57.698, 150.445
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.850 - 2.600
R-factor	0.1878
Rwork	0.186
R-free	0.21600
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.435
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	8435	588
<I/σ(I)>	22.4
Completeness [%]	100.0
Redundancy	323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.7 M SODIUM TARTRATE, 100 MM ADA, PH6.5