6O7C
Crystal structure of the LjCASTOR gating ring in the Ca2+ and K+ state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99996 |
Spacegroup name | I 4 |
Unit cell lengths | 125.569, 125.569, 82.787 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.710 - 1.850 |
R-factor | 0.1747 |
Rwork | 0.173 |
R-free | 0.20960 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.728 |
Data reduction software | HKL-2000 (HKL2000_v712-Linux) |
Data scaling software | HKL-2000 (HKL2000_v712-Linux) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.063 | 1.670 |
Rmeas | 0.068 | 1.799 |
Rpim | 0.025 | 0.668 |
Number of reflections | 55045 | 2759 |
<I/σ(I)> | 23.4 | 1 |
Completeness [%] | 99.7 | |
Redundancy | 7.4 | 7.2 |
CC(1/2) | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 21% w/v PEG550 MME, 50 mM magnesium acetate, 100 mM Tris, pH 8.2 |