6O7A
Crystal structure of the LjCASTOR gating ring in the Ca2+-free state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 100.433, 116.015, 113.027 |
Unit cell angles | 90.00, 113.92, 90.00 |
Refinement procedure
Resolution | 41.470 - 3.300 |
R-factor | 0.2321 |
Rwork | 0.229 |
R-free | 0.28410 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.776 |
Data reduction software | HKL-2000 (hkl2000 v712-Linux) |
Data scaling software | HKL-2000 (hkl2000 v712-Linux) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.083 | 0.548 |
Rmeas | 0.096 | 0.637 |
Rpim | 0.049 | 0.324 |
Number of reflections | 35911 | 1776 |
<I/σ(I)> | 18.9 | 2.91 |
Completeness [%] | 99.7 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% w/v PEG3350, 100 mM lithium acetate |