6O76
Human cytosolic Histidyl-tRNA synthetase (HisRS) with WHEP domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2012-06-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 97.669, 97.669, 254.409 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.021 - 2.787 |
R-factor | 0.2388 |
Rwork | 0.236 |
R-free | 0.29520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x5o |
RMSD bond length | 0.008 |
RMSD bond angle | 1.424 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.870 |
High resolution limit [Å] | 2.787 | 2.787 |
Number of reflections | 31689 | 1954 |
<I/σ(I)> | 16.4 | |
Completeness [%] | 97.0 | |
Redundancy | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.29 mM potassium sodium tartrate tetrahydrate, 18% PEG3350 |