6O6Z

Crystal structure of Csm6 H381A in complex with cA4 by cocrystallization of cA4 and Csm6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	54.292, 115.940, 159.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.350 - 2.100
R-factor	0.19394
Rwork	0.192
R-free	0.22155
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o6z
RMSD bond length	0.010
RMSD bond angle	1.728
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rpim	0.055
Number of reflections	59191	5719
<I/σ(I)>	16.9
Completeness [%]	99.4
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	10% PEG 8K, 0.2 M NaCl, 0.1 M Na/K phosphate pH 6.2