6O6L
The Structure of EgtB(Cabther) in complex with Hercynine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2016-09-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97648 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.164, 137.513, 85.218 |
Unit cell angles | 90.00, 92.37, 90.00 |
Refinement procedure
Resolution | 45.827 - 2.250 |
R-factor | 0.1881 |
Rwork | 0.187 |
R-free | 0.22090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.969 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 92240 | 6085 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 99.6 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | 0.2M Na Acetate, 0.1M Tris Acetate pH 6.5 and 5% PEG 4000 |