6O6K

HUaa 19bp SYM DNA pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.115830
Spacegroup name	P 42 21 2
Unit cell lengths	84.790, 84.790, 63.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.956 - 3.601
R-factor	0.2571
Rwork	0.252
R-free	0.30190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4YEX and DNA
RMSD bond length	0.003
RMSD bond angle	0.525
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.789	3.730
High resolution limit [Å]	2.994	3.602
Rmerge	0.135
Rmeas	0.141
Number of reflections	2939	18426
<I/σ(I)>	15.1
Completeness [%]	99.7	98.6
Redundancy	13.8	12.9
CC(1/2)	1.000	0.245

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.02M ZnCl2, 20% PEG 3350