6O6H
RIAM cc-RA-PH structure in the P21212 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97750 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.146, 92.981, 44.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.390 - 2.500 |
R-factor | 0.23129 |
Rwork | 0.229 |
R-free | 0.28092 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.359 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.390 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 12656 | 603 |
<I/σ(I)> | 20.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.2 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM sodium malonate pH 6.0, 16% PEG 3350, 3% 1,4-dioxane |