6O6D

N-terminal domain of translation initiation factor IF-3 from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-27
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	56.468, 56.468, 55.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.480 - 1.820
R-factor	0.1769
Rwork	0.175
R-free	0.21450
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.691
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.480	39.480	1.850
High resolution limit [Å]	1.820	4.940	1.820
Rmerge	0.059	0.050	0.934
Rmeas	0.063	0.054	0.998
Rpim	0.021	0.019	0.339
Number of reflections	8462	489	397
<I/σ(I)>	15.5		1.75
Completeness [%]	99.8	97.6	99.5
Redundancy	8.3	7.1	7.7
CC(1/2)	0.997	0.997	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.09 M HEPES-NaOH buffer, 1.26 M sodium citrate, 10% glycerol