6O6D
N-terminal domain of translation initiation factor IF-3 from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-27 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 56.468, 56.468, 55.142 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.480 - 1.820 |
R-factor | 0.1769 |
Rwork | 0.175 |
R-free | 0.21450 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.691 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.480 | 39.480 | 1.850 |
High resolution limit [Å] | 1.820 | 4.940 | 1.820 |
Rmerge | 0.059 | 0.050 | 0.934 |
Rmeas | 0.063 | 0.054 | 0.998 |
Rpim | 0.021 | 0.019 | 0.339 |
Number of reflections | 8462 | 489 | 397 |
<I/σ(I)> | 15.5 | 1.75 | |
Completeness [%] | 99.8 | 97.6 | 99.5 |
Redundancy | 8.3 | 7.1 | 7.7 |
CC(1/2) | 0.997 | 0.997 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.09 M HEPES-NaOH buffer, 1.26 M sodium citrate, 10% glycerol |