6O5L
Crystal structure of PprA filament from Deinococcus peraridilitoris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 79.468, 79.468, 262.804 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 66.576 - 3.300 |
R-factor | 0.2489 |
Rwork | 0.247 |
R-free | 0.29860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mc8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.059 |
Data reduction software | iMOSFLM (7.2.2) |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER ((1.13_2998: ???)) |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.820 | 3.560 |
High resolution limit [Å] | 3.300 | 3.300 |
Rpim | 0.075 | |
Number of reflections | 8106 | 1591 |
<I/σ(I)> | 10.7 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 32.9 | 35.4 |
CC(1/2) | 0.999 | 0.507 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293.15 | 54 uM protein, 5% w/v PEG8000, 500 mM sodium chloride, 75 mM imidazole, 10 mM Tris, 50 mM potassium phosphate, 10 mM trimethylamine-HCl |