6O5K

Murine TRIM28 Bbox1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	33.012, 51.459, 72.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.040 - 1.600
R-factor	0.18181
Rwork	0.180
R-free	0.20764
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.194
Data reduction software	XDS (Jan 10, 2014)
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.040	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.267	3.950
Rpim	0.072	1.075
Number of reflections	17060	817
<I/σ(I)>	10.3	0.97
Completeness [%]	100.0	100
Redundancy	14.3	14.2
CC(1/2)	0.997	0.391

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	291	2.7 M Ammonium sulfate, pH 6.0