6O5H
The effect of modifier structure on the activation of leukotriene A4 hydrolase aminopeptidase activity.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-05-23 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 |
Unit cell lengths | 139.663, 139.663, 84.149 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 120.950 - 2.840 |
R-factor | 0.2191 |
Rwork | 0.219 |
R-free | 0.22860 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.951 |
Data reduction software | PROTEUM |
Data scaling software | PROTEUM |
Phasing software | PROTEUM |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 120.950 | 2.914 |
High resolution limit [Å] | 2.840 | 2.840 |
Number of reflections | 40540 | |
<I/σ(I)> | 4.02 | |
Completeness [%] | 98.6 | |
Redundancy | 6.72 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 50 - 80 mM magnesium formate dihydrate and 24% - 29% PEG3350 |