6O5C
X-ray crystal structure of metal-dependent transcriptional regulator MtsR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 67.904, 67.904, 293.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.738 - 3.100 |
| R-factor | 0.2474 |
| Rwork | 0.245 |
| R-free | 0.28620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cvi |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.594 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 23865 | 3461 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 40 mM sodium acetate, 0.16 M magnesium formate, 2.6 M lithium acetate, 20% PEG 2000, 66 mM ammonium sulfate, and 0.8 mM manganese chloride |
