6O59

Crystal structure of the N-terminal domain of the A subunit of the Bacillus megaterium spore germinant receptor GerK3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 41 21 2
Unit cell lengths	106.245, 106.245, 140.188
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.790
R-factor	0.206
Rwork	0.205
R-free	0.22700
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.170
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	RESOLVE
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.790	2.790
Rmerge		0.821
Number of reflections	20443
<I/σ(I)>	21
Completeness [%]	99.5
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1 M Tris, pH 8.5, 0.15 M lithium sulfate, 10-15% PEG6000
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1 M Tris, pH 8.5, 0.15 M lithium sulfate, 10-15% PEG6000