6O40
Human parainfluenza virus type 3 fusion protein N-terminal heptad repeat domain+VIQKI I454F I456F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | H 3 |
Unit cell lengths | 49.337, 49.337, 75.894 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.370 - 1.200 |
R-factor | 0.1765 |
Rwork | 0.175 |
R-free | 0.18550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nro |
RMSD bond length | 0.011 |
RMSD bond angle | 1.363 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.370 | 1.240 |
High resolution limit [Å] | 1.197 | 1.197 |
Rmerge | 0.042 | 0.714 |
Rmeas | 0.044 | 0.828 |
Rpim | 0.013 | 0.405 |
Number of reflections | 21440 | 1943 |
<I/σ(I)> | 21.65 | 1.55 |
Completeness [%] | 98.4 | 88.36 |
Redundancy | 9.8 | 3.8 |
CC(1/2) | 1.000 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 30 mM NaF, 30 mM NaBr, 30 mM NaI, 20% (v/v) PEG 500 MME, 10% (w/v) PEG 20000 in 100 mM imidazole/MES monohydrate buffer (pH 6.5)20% (v/v) PEG 500 MME, 10% (w/v) PEG 20000 in 100 mM imidazole/MES monohydrate buffer (pH 6.5) |