6O3V

Crystal structure for RVA-VP3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	CCD
Collection date	2017-08-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9999
Spacegroup name	P 62 2 2
Unit cell lengths	236.706, 236.706, 349.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.175 - 3.500
R-factor	0.2307
Rwork	0.229
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.871
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3235: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.175	3.625
High resolution limit [Å]	3.500	3.500
Rpim	0.052
Number of reflections	71574	5807
<I/σ(I)>	4.5
Completeness [%]	97.7
Redundancy	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	Protein conc ~ 7 mg/ml 0.2 M Lithium Sulphate 0.1 M Sodium citrate pH 5.5 15% Ethanol