6O3A

Crystal structure of Frizzled 7 CRD in complex with F7.B Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-01
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	41.544, 103.446, 144.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.551 - 2.100
R-factor	0.1943
Rwork	0.192
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bpq
RMSD bond length	0.007
RMSD bond angle	0.940
Data reduction software	XPREP
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.551	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.124	1.120
Rpim	0.049	0.438
Number of reflections	37237
<I/σ(I)>	12.6
Completeness [%]	99.9
Redundancy	7.3
CC(1/2)	0.998	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	10	293	0.1 M CAPS, pH 10.5, 20% (w/v) PEG 3350, 0.2 M NaCl